Part:BBa_C2001:Design

Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 319
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 79
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This protein should form a dimer and bind a specific operator site, thus repressing transcription.

Source

Protein Engineering Gods from the Pabo Lab